Review



dii fluorescent cell linker kit  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Beyotime dii fluorescent cell linker kit
    Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and <t>fluorescent</t> images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant
    Dii Fluorescent Cell Linker Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dii+fluorescent+cell+linker+kit/DiI/pmc12913794-480-23-28
    Average 99 stars, based on 1021 article reviews
    dii fluorescent cell linker kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis"

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis

    Journal: Bone Research

    doi: 10.1038/s41413-025-00502-4

    Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and fluorescent images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant
    Figure Legend Snippet: Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and fluorescent images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant

    Techniques Used: Derivative Assay, Injection, Staining, Drug discovery, Expressing, Immunohistochemistry

    Related Articles

    Derivative Assay:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Injection:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Staining:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Drug discovery:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Expressing:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Immunohistochemistry:

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis
    Article Snippet: The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).The ultrastructure of EVs was further examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan).. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis.
    Article Snippet: examined using transmission electron microscopic (TEM), and images were acquired with Hitachi HT7800 electron microscope (HT-7800, Hitachi, Japan). .. For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C. .. Lastly, DiI-labeled EVs were reconstituted in PBS for the following investigation with chondrocytes.



    Similar Products

    dii fluorescent cell linker kit  (Beyotime)
    99
    Beyotime dii fluorescent cell linker kit
    Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and <t>fluorescent</t> images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant
    Dii Fluorescent Cell Linker Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dii+fluorescent+cell+linker+kit/DiI/pmc12913794-480-23-28
    Average 99 stars, based on 1 article reviews
    dii fluorescent cell linker kit - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and fluorescent images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant

    Journal: Bone Research

    Article Title: Synovial inflammatory macrophage-derived extracellular vesicles exacerbate cartilage lesions with a FMRP-selectively sorted manner in osteoarthritis

    doi: 10.1038/s41413-025-00502-4

    Figure Lengend Snippet: Engineering ADSCs-derived EVs promote cartilage repairment in OA model. a Scheme showing the design of engineering ADSCs-derived EVs. b Schematic illustration of the animal experimental procedure. EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p were intra-articular injected into OA rats, and the joint samples were collected after 12 h. c IF staining of synovial pro-inflammatory macrophages and fluorescent images of synovium tissues treated with EVs ADSCs or MAP-EVs ADSCs loading with FAM-antagomiR-155-5p. scale bar: 100 μm. d A schematic diagram to evaluate the therapeutic efficacy of engineering EVs ADSCs in OA rats model. Representative images ( e ) and quantitative analysis ( f ) of SO & FG staining in keen joints of sham or OA rats treated with PBS, EVs ADSCs , EVs ADSCs -antagomiR-155-5p and MAP-EVs ADSCs -antagomiR-155-5p. (up), scale bar: 500 μm. Higher magnification images show dramatic articular cartilage changes (down), scale bar: 100 μm. n = 7 for each group. Representative images ( g ) and quantitative analysis ( j ) of IF staining of knee joint sections showing expression of COL2A1 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. DAPI: 4,6-diamidino-2-phenylindole. n = 7 for each group. Representative images ( h ) and quantitative analysis ( k ) of IHC staining of knee joint sections showing expression of MMP13 in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. Representative images ( i ) and quantitative analysis ( l ) of IHC staining of knee joint sections showing expression of GSK-3β in articular cartilage of the aforementioned rats treated with engineering EVs. Scale bar: 100 μm. n = 6 for each group. One-way ANOVA &Tukey HSD post hoc test (normal distribution) and Kruskal-Wallis Test & Dunn’s test (non-normal distribution) were used for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.000 1, ns not significant

    Article Snippet: For co-culture investigations of chondrocytes and BMDMs-EVs, the EVs recovered from the BMDMs culture supernatant were dyed with DiI fluorescent dye via the DiI fluorescent cell linker kit (Beyotime, C1991S), followed by rinsing in PBS and ultracentrifugation (100 000 × g for 70 min) at 4 °C.

    Techniques: Derivative Assay, Injection, Staining, Drug discovery, Expressing, Immunohistochemistry